Journal: Frontiers in Genetics

Article Title: Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models

doi: 10.3389/fgene.2021.615491

Figure Lengend Snippet: Resources on rat genomics and genome edited animals.

Article Snippet: , Cyagen , https://www.cyagen.com/us/en/ , Custom rat model generation.

Techniques: Expressing, Sequencing, Variant Assay, Comparison, Software, Transgenic Assay, CRISPR

Journal: Frontiers in Genetics

Article Title: Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models

doi: 10.3389/fgene.2021.615491

Figure Lengend Snippet: Rat research model generation by CRISPR-Cas9 and applications. Strategies to generate research models by CRISPR-cas9 are multiple and very helpful for studies of gene function and diseases or to generate a reporter model. (A) The RNP alone can be used to create indels at one or more loci to generate single or multiple KO or a large deletion. (B) RNP with a short DNA donor (ssODN) can be used to generate a stop codon or mutations or to insert a Tag in the reading frame of the endogenous gene of interest. (C) A large DNA donor (either lsDNA, dsDNA, or plasmid) can be used to express a reporter gene in the reading frame of the endogenous targeted gene with a self-cleaving peptide, to generate conditional or inducible Cre/lox models with or without a reporter, or to overexpress the rat or human gene of interest or a reporter gene in a safe harbor locus. For expression of inserted genes, an endogenous or ubiquitous promoter or a specific promoter can be used to restrict expression to tissues or cell types. Figure created with BioRender.com . SNP, single nucleotide polymorphism; RNP, ribonucleoprotein complex; 2A, self-cleaving peptide; KO, knockout; indels, insertion or deletion; Cre, Cre recombinase.

Article Snippet: , Cyagen , https://www.cyagen.com/us/en/ , Custom rat model generation.

Techniques: CRISPR, Plasmid Preparation, Expressing, Knock-Out